赵云龙^1，2，火红艳³，金 燕^4，5*，赵四标^1，2

（1.昆明市食品药品检验所，云南昆明 650032；

2.昆明市食品药品检验研究院，云南昆明 650032；

3.玉溪市第二人民医院，云南玉溪 653100；

4.云南省第一人民医院药学部，云南昆明 650032；

5.昆明理工大学附属医院药学部，云南昆明 650032）

摘要：建立大白菜中阿维菌素残留量的准确、快速检测方法。采用QuECHERS-高效液相紫外法测定大白菜中的阿维菌素，用乙腈和萃取试剂（MgSO₄、NaCl、柠檬酸盐混合物）提取试样，漩涡振荡、离心；再通过PSA、GCB、MgSO₄净化，漩涡振荡、离心；最后收集经0.22 μm有机滤膜过滤的滤液上机检测。在阿维菌素质量浓度0.1～10.0 mg/L范围内，线性关系较好，相关系数 R²＝0.999 98。0.2 mg/L、2.0 mg/L、4.0 mg/L、10.0 mg/L 4 组阿维菌素质量浓度测定的重复性好，RSD 在0.03%～0.28%之间。空白样品试样在0.2mg/L、0.4mg/L、0.5mg/L、1.0mg/L 4个浓度添加水平的回收率为81.35%～100.67%。该方法操作简单、方便快捷、灵敏度高、重复性好、稳定性好、结果满意，适用于大白菜中阿维菌素的准确、快速检测；高效液相色谱仪配紫外检测器的普及率高，该方法具有较强的操作性和基层推广意义。

关键词：高效液相色谱仪，大白菜，阿维菌素，残留量

中图分类号：TS207.3  文献标识码：A  文章编号：1674-506X（2021）06-0109-0005

QuECHERS-HPLC Determination of Abamectin Residues in Chinese Cabbage

ZHAO Yun-long^1，2, HUO Hong-yan³, JIN Yan^4，5*, ZHAO Si-biao^1，2

（1.Kunming Institute for Food and Drug Control, Kunming Yunnan 650032, China;

2.Kunming Institute of Food and Drug Inspection, Kunming Yunnan 650032, China;

3.The Second People’s Hospital of Yuxi, Yuxi Yunnan 653100, China;

4.The First People’s Hospital of Yunnan Province, Kunming Yunnan 650032, China;

5.The Affiliated Hospital of Kunming University of Science and Technology, Kunming Yunnan 650032, China）

Abstract：To establish an accurate and rapid method for the determination of abamectin residue in Chinese cabbage. The QuECHERS-high performance liquid (HPLC)-UV method was used to determine the abamectin in Chinese cabbage. The sample was extracted with acetonitrile and extraction reagent (the mixture of MgSO₄, NaCl and citrate), vortexed, centrifuged; and then the sample was purified by PSA, GCB and MgSO₄, vortexed, centrifuged; and finally collect the filtrate filtered through a 0.22 μm organic filter membrane for machine testing. In the range of abamectin mass concentration 0.1～10.0 mg/L, the linear relationship was good, and the correlation coefficient R²＝0.999 98. The reproducibility of abamectin mass concentration in the 4 groups of 0.2 mg/L, 2.0 mg/L, 4.0 mg/L and 10.0 mg/L was good, and the RSD is between 0.03% and 0.28%. The recovery rate of blank samples were 81.35%～100.67% at the four supplemental levels of 0.2 mg/L, 0.4 mg/L, 0.5 mg/L and 1.0 mg/L. The method is simple, convenient, sensitive, repeatable, stable and satisfactory. It 0is suitable for the accurate and rapid determination of abamectin in Chinese cabbage. HPLC with ultraviolet detector has a high popularity rate, the method has strong operability and basic promotion significance.

Keywords：high performance liquid chromatography (HPLC); Chinese cabbage; abamectin; residual

doi：10.3969/j.issn.1674-506X.2021.06-018

收稿日期：2021-05-07

作者简介：赵云龙（1984-），男，本科，工程师。研究方向：食品检验检测。

*通信作者

引文格式：赵云龙,火红艳,金燕,等.QuECHERS-HPLC测定大白菜中阿维菌素残留量[J].食品与发酵科技,2021,57(6):109-112,131.